Selected Publications
1. Melamed, P., Savulescu, D., Lim, S., Wijeweera, A., Luo, Z.J., Luo, M., Pnueli, L. 2012. Gonadotropin releasing hormone (GnRH)-signalling downstream of calmodulin. J. Neuroendocrinol. 24:1463-75.
2. Pnueli, L., Luo, M., Wang, S., Naor, Z. and Melamed, P. 2011. Calcineurin regulates expression of both subunits of the Follicle-Stimulating Hormone through distinct mechanisms. Mol. Cell. Biol. 31:5023-36.
3. Melamed, P. (2010) Hormonal signaling to FSHβ gene expression. Mol. Cell. Endocrinol. 314:204-12.
4. Luo, Z.J., Wijeweera, A., Oh, Y.Z., Liou, Y.C. and Melamed, P. 2010. Pin1 regulates gonadotropin β-subunit gene transcription through facilitating the phosphorylation-dependent ubiquitination of SF-1. Mol. Cell. Biol. 30:745-63.
5. Dobkin-Bekman, M., Naidich, M., Rahamim, L., Przedecki, F., Almog, T., Lim, S., Melamed, P., Liu, P., Wohland, T., Yao, Z., Seger, R. and Naor Z. 2009. A preformed signaling complex mediates GnRH activated ERK phosphorylation of paxillin and FAK at focal adhesions in LβT2 gonadotrope cells. Mol. Endocrinol. 23:1850-64.
6. Lim, S., Pnueli, L., Tan, J.H., Naor Z., Rajagopal, G. and Melamed P. 2009. Negative feedback governs gonadotrope frequency-decoding of gonadotropin releasing hormone pulse-frequency PLoS One 4:e7244
7. Wang, S., Zhu, Y. and Melamed, P. 2009. The molecular regulation of Chinook salmon gonadotropin β-subunit gene transcription. Gen. Comp. Endocrinol. 161:34-41.
8. Feng, J. Lawson, M.A. and Melamed, P. 2008. A proteomic comparison of immature and mature mouse gonadotrophs reveals novel differentially expressed nuclear proteins that regulate gonadotropin gene transcription and RNA splicing. Biol. Reprod. 79:546-61.
9. Melamed, P. 2008. The role of histone deacetylases in expression of gonadotropin subunit genes. Trends Endocrinol. Metab. 19:25-31.
10. Lim, S., Luo, M., Koh, M., Yang, M., Abdul Kadir, M.N., Tan, J.H., Ye, Z., Wang, W. and Melamed, P. 2007. Distinct mechanisms involving diverse histone deacetylases repress expression of the two gonadotropin β-subunit genes in immature gonadotropes, and their actions are overcome by GnRH. Mol. Cell. Biol. 27:4105-20.
11. Melamed, P. Abdul Kadir, M.N., Wijeweera, A. and Seah, S. 2006. Transcription of gonadotropin β subunit genes involves cross-talk between the transcription factors and co-regulators that mediate actions of the regulatory hormones. Mol. Cell. Endocrinol. 252:167-83.
12. Melamed, P., Zhu, Y., Tan, S.H., Xie, M. and Koh. M. 2006. Gonadotropin releasing hormone activation of c-jun, but not Egr-1, stimulates transcription of a luteinizing hormone β subunit gene. Endocrinology 147:3598-605.
13. Luo, M., Koh, M., Feng, J., Wu, Q. and Melamed, P. 2005. Cross-talk in hormonally-regulated gene transcription through induction of estrogen receptor ubiquitylation. Mol. Cell. Biol. 25:7386-98.
14. Melamed, P., Xue, Y.K., Poon, J.F., Wu, Q., Xie H., Yeo, J., Foo, T.W. and Chua H.K. (2005) The male seahorse synthesizes and secretes a novel C-type lectin into the brood pouch during early pregnancy. FEBS J. 272:1221-35.
15. Chong, K.L. Wang, S. and Melamed, P. 2004. Isolation and characterization of the follicle-stimulating hormone β subunit gene and 5’ flanking region of the Chinook salmon. Neuroendocrinology 80:158-70.
16. Melamed, P., Chong, K.L. and Johansen, M.V. 2004. Evidence for lateral gene transfer from salmonids to two Schistosome species. Nature Genetics 36:786-7.
17. Yaron, Z., Gur, G., Melamed, P., Rosenfeld, H. and Elizur, A. and Levavi-Sivan, B. 2003. Regulation of fish gonadotropins. Int. Rev. Cytol. 225:131-85.
18. Melamed, P., Koh, M., Preklathan, P., Bei, L. and Hew, C.L. 2002. Multiple mechanisms for Pitx-1 transactivation of a luteinizing hormone β subunit gene. J. Biol. Chem. 277:26200-07.
1. Melamed, P., Savulescu, D., Lim, S., Wijeweera, A., Luo, Z.J., Luo, M., Pnueli, L. 2012. Gonadotropin releasing hormone (GnRH)-signalling downstream of calmodulin. J. Neuroendocrinol. 24:1463-75.
2. Pnueli, L., Luo, M., Wang, S., Naor, Z. and Melamed, P. 2011. Calcineurin regulates expression of both subunits of the Follicle-Stimulating Hormone through distinct mechanisms. Mol. Cell. Biol. 31:5023-36.
3. Melamed, P. (2010) Hormonal signaling to FSHβ gene expression. Mol. Cell. Endocrinol. 314:204-12.
4. Luo, Z.J., Wijeweera, A., Oh, Y.Z., Liou, Y.C. and Melamed, P. 2010. Pin1 regulates gonadotropin β-subunit gene transcription through facilitating the phosphorylation-dependent ubiquitination of SF-1. Mol. Cell. Biol. 30:745-63.
5. Dobkin-Bekman, M., Naidich, M., Rahamim, L., Przedecki, F., Almog, T., Lim, S., Melamed, P., Liu, P., Wohland, T., Yao, Z., Seger, R. and Naor Z. 2009. A preformed signaling complex mediates GnRH activated ERK phosphorylation of paxillin and FAK at focal adhesions in LβT2 gonadotrope cells. Mol. Endocrinol. 23:1850-64.
6. Lim, S., Pnueli, L., Tan, J.H., Naor Z., Rajagopal, G. and Melamed P. 2009. Negative feedback governs gonadotrope frequency-decoding of gonadotropin releasing hormone pulse-frequency PLoS One 4:e7244
7. Wang, S., Zhu, Y. and Melamed, P. 2009. The molecular regulation of Chinook salmon gonadotropin β-subunit gene transcription. Gen. Comp. Endocrinol. 161:34-41.
8. Feng, J. Lawson, M.A. and Melamed, P. 2008. A proteomic comparison of immature and mature mouse gonadotrophs reveals novel differentially expressed nuclear proteins that regulate gonadotropin gene transcription and RNA splicing. Biol. Reprod. 79:546-61.
9. Melamed, P. 2008. The role of histone deacetylases in expression of gonadotropin subunit genes. Trends Endocrinol. Metab. 19:25-31.
10. Lim, S., Luo, M., Koh, M., Yang, M., Abdul Kadir, M.N., Tan, J.H., Ye, Z., Wang, W. and Melamed, P. 2007. Distinct mechanisms involving diverse histone deacetylases repress expression of the two gonadotropin β-subunit genes in immature gonadotropes, and their actions are overcome by GnRH. Mol. Cell. Biol. 27:4105-20.
11. Melamed, P. Abdul Kadir, M.N., Wijeweera, A. and Seah, S. 2006. Transcription of gonadotropin β subunit genes involves cross-talk between the transcription factors and co-regulators that mediate actions of the regulatory hormones. Mol. Cell. Endocrinol. 252:167-83.
12. Melamed, P., Zhu, Y., Tan, S.H., Xie, M. and Koh. M. 2006. Gonadotropin releasing hormone activation of c-jun, but not Egr-1, stimulates transcription of a luteinizing hormone β subunit gene. Endocrinology 147:3598-605.
13. Luo, M., Koh, M., Feng, J., Wu, Q. and Melamed, P. 2005. Cross-talk in hormonally-regulated gene transcription through induction of estrogen receptor ubiquitylation. Mol. Cell. Biol. 25:7386-98.
14. Melamed, P., Xue, Y.K., Poon, J.F., Wu, Q., Xie H., Yeo, J., Foo, T.W. and Chua H.K. (2005) The male seahorse synthesizes and secretes a novel C-type lectin into the brood pouch during early pregnancy. FEBS J. 272:1221-35.
15. Chong, K.L. Wang, S. and Melamed, P. 2004. Isolation and characterization of the follicle-stimulating hormone β subunit gene and 5’ flanking region of the Chinook salmon. Neuroendocrinology 80:158-70.
16. Melamed, P., Chong, K.L. and Johansen, M.V. 2004. Evidence for lateral gene transfer from salmonids to two Schistosome species. Nature Genetics 36:786-7.
17. Yaron, Z., Gur, G., Melamed, P., Rosenfeld, H. and Elizur, A. and Levavi-Sivan, B. 2003. Regulation of fish gonadotropins. Int. Rev. Cytol. 225:131-85.
18. Melamed, P., Koh, M., Preklathan, P., Bei, L. and Hew, C.L. 2002. Multiple mechanisms for Pitx-1 transactivation of a luteinizing hormone β subunit gene. J. Biol. Chem. 277:26200-07.
MOLECULAR ENDOCRINOLOGY LAB
A beautiful fish pituitary (Melamed et al., GCE 1995)
Gonadotrope nuclear proteomes
